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Tarak El-Bialy, et al.
Ultrasound Tooth Regeneration
https://www.scirp.org/journal/paperinformation?paperid=29409
Open Journal of Regenerative Medicine, Vol.2 No.1, March 2013
Effects of low intensity pulsed ultrasound stimulation on bone regeneration in rat parietal bone defect model
Kang Chen, Jia Hao, Kanako Noritake, Yu Yamashita, Shinji Kuroda, Shohei Kasugai
Abstract -- Purpose: Low intensity pulsed ultrasound stimulation (LIPUS) has been clinically applied to promote bone fracture healing in the orthopedic field. Thus, it is likely that LIPUS also stimulates bone regeneration in bone defects in the cranial-maxillofacial area. However, this has not been clearly proved. Furthermore, optimal time point and period of the application after the surgery has not been reported. The purpose of the present study was to evaluate the effect of LIPUS on bone regeneration in the rat parietal bone defects especially focusing on time and period of the application. Materials and Methods: Eighteen Wistar rats (14 weeks old) were divided into 6 groups: 5 experimental groups and a control group. Bone defect of 5 mm diameter was prepared on each side of the parietal bone and customized gelatin membranes were placed over the bone defects. LIPUS (160 mW/cm2, 15 min/day) was applied to the defect area with an active transducer externally in the experimental groups according to the schedules of the applications: Group 1 (day 6 - 12), group 2 (day 13 - 19), group 3 (day 20 - 26), group 4 (day 6 - 19) and group 5 (day 6 - 26). All the animals were sacrificed at 28 days. The defects were analyzed with micro CT and then histologically. Results: In Group 1, new bone formation was significantly promoted and the newly-formed bone was thick and matured compared to the one of the control group. In other experimental groups there were tendencies of stimulation of new bone formation; however, they were not statistically significant. Discussion and Conclusion: The present study demonstrated that amount of new bone formation in the bone defect depended on the time and period of LIPUS application. It has been suggested that application of LIPUS at an early healing period, the second week after the surgery, effectively accelerated new bone formation.
https://www.nature.com/articles/4813802
British Dental Journal volume 201, page 9 (2006)
Ultrasound may help regrow teeth
A team of researchers from the University of Alberta has created technology to regrow teeth. Using low-intensity pulsed ultrasound (LIPUS), Dr Tarak El-Bialy from the Faculty of Medicine and Dentistry together with Dr Jie Chen and Dr Ying Tsui from the Faculty of Engineering have created a miniaturised system-on-a-chip that offers a non-invasive and novel way to stimulate jaw growth and dental tissue healing...
https://www.ualberta.ca/en/folio/2020/10/daily-use-of-ultrasound-can-speed-up-orthodontic-treatment.html
Daily use of ultrasound can speed up orthodontic treatment, prevent root damage: studies
By Gillian Rutherford
U of A orthodontist and engineers team up to create and patent new dental device...
https://www.mdpi.com/2077-0383/9/5/1303
Shortening of Overall Orthodontic Treatment Duration with Low-Intensity Pulsed Ultrasound (LIPUS)
by Harmanpreet Kaur, Tarek El-Bialy
Abstract -- The aim of this retrospective clinical study was to determine if there is a reduction in the overall treatment duration in orthodontic patients using low-intensity pulsed ultrasound (LIPUS) and Invisalign SmartTrack® clear aligners. Data were collected from the first thirty-four patients (9 males, 25 females; average age 41.37 ± 15.02) who finished their orthodontic treatment using an intraoral LIPUS device and Invisalign clear aligners in a private clinic. The LIPUS parameters used by patients at home for 20 min/day were: ultrasonic frequency 1.5 MHz, pulse duration 200µs, pulse repetition rate 1 kHz, and spatial average-temporal average intensity 30mW/cm2. A control group (11 males, 23 females; average age 31.36 ± 14.41) matching for the same malocclusions was randomly selected from finished treatment cases of the same clinician. The date of first Invisalign attachment placement and first use of LIPUS application was recorded as T0, and the date of retainer delivery was recorded as T1. The treatment duration (T1–T0) and treatment reduction percentage with LIPUS device were collected and analyzed using two-sample t-test in Microsoft Excel. Treatment duration was significantly reduced in the LIPUS group (541.44 ± 192.23 days) compared to control group (1061.05 ± 455.64 days) (p < 0.05). The LIPUS group showed on average 49% reduction in the overall treatment time as compared to the control group. The average compliance of the patients using LIPUS was 66.02%. Patients who used LIPUS showed a clinically significant reduction in the overall orthodontic treatment duration compared to the control group who used Invisalign clear aligners only.
https://pmc.ncbi.nlm.nih.gov/articles/PMC3547311/
Dent J. 2012 Dec 28;6:220–225.
doi: 10.2174/1874210601206010220
Current Status of Low Intensity Pulsed Ultrasound for Dental Purposes
Emanuel Braga Rego, Takashi Takata, Kazuo Tanne, Eiji Tanaka
Abstract -- Over the past few years, tissue engineering applied to the dental field has achieved relevant results. Tissue engineering can be described by actions taken to improve biological functions. Several methods have been described to enhance cellular performance and low intensity pulsed ultrasound (LIPUS) has shown to play an important role in cell metabolism. The present article provides an overview about the current status of LIPUS as a tissue engineering tool to be used to enhance tooth and periodontal regeneration.
https://www.sciencedirect.com/science/article/abs/pii/S0306987709004009
Therapeutic ultrasound for dental tissue repair
Author links open overlay panelB.A.A. Scheven
, R.M. Shelton, P.R. Cooper, A.D. Walmsley, A.J. Smith
Summary -- Dental disease affects human health and the quality of life of millions worldwide. Tooth decay (caries) and diseases of the dental pulp result in loss of tooth vitality and function requiring invasive treatment to restore the tooth to health. “Therapeutic” low intensity pulsed ultrasound has been shown to accelerate bone fracture healing indicating that ultrasound may be used as a tool to facilitate hard tissue regeneration. We have shown recently that low frequency ultrasound is able to exert biological effects on odontoblast-like cells. In this paper, we postulate that low frequency, low intensity ultrasound may stimulate endogenous coronal tooth repair by stimulating dentine formation from existing odontoblasts or by activating dental pulp stem cells to differentiate into new reparative dentine-producing cells. Ultrasound therapy promoting dentine formation and repair may also have the potential benefit of alleviating dentine hypersensitivity by inducing occlusion of dentinal tubules. It is envisaged that therapeutic ultrasound may be used in future to facilitate dental tissue engineering and stem cell therapy applications for dental tissue regeneration. Further research is warranted in this clinically important area and we envisage that novel strategies in dental therapy will be realised that may ultimately lead to the development of novel non-invasive, multifunctional ultrasound devices for dental diagnostics, repair and regeneration.
https://pubmed.ncbi.nlm.nih.gov/23853309/
PMID: 23853309 DOI: 10.1109/TBCAS.2009.2034635
Design and implementation of therapeutic ultrasound generating circuit for dental tissue formation and tooth-root healing
Woon Tiong Ang, C Scurtescu, Wing Hoy, T El-Bialy, Ying Yin Tsui, Jie Chen
Abstract -- Biological tissue healing has recently attracted a great deal of research interest in various medical fields. Trauma to teeth, deep and root caries, and orthodontic treatment can all lead to various degrees of root resorption. In our previous study, we showed that low-intensity pulsed ultrasound (LIPUS) enhances the growth of lower incisor apices and accelerates their rate of eruption in rabbits by inducing dental tissue growth. We also performed clinical studies and demonstrated that LIPUS facilitates the healing of orthodontically induced teeth-root resorption in humans. However, the available LIPUS devices are too large to be used comfortably inside the mouth. In this paper, the design and implementation of a low-power LIPUS generator is presented. The generator is the core of the final intraoral device for preventing tooth root loss and enhancing tooth root tissue healing. The generator consists of a power-supply subsystem, an ultrasonic transducer, an impedance-matching circuit, and an integrated circuit composed of a digital controller circuitry and the associated driver circuit. Most of our efforts focus on the design of the impedance-matching circuit and the integrated system-on-chip circuit. The chip was designed and fabricated using 0.8- ¿m high-voltage technology from Dalsa Semiconductor, Inc. The power supply subsystem and its impedance-matching network are implemented using discrete components. The LIPUS generator was tested and verified to function as designed and is capable of producing ultrasound power up to 100 mW in the vicinity of the transducer's resonance frequency at 1.5 MHz. The power efficiency of the circuitry, excluding the power supply subsystem, is estimated at 70%. The final products will be tailored to the exact size of teeth or biological tissue, which is needed to be used for stimulating dental tissue (dentine and cementum) healing.
https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2022.961898/full
Low-intensity pulsed ultrasound promotes periodontal regeneration in a beagle model of furcation involvement
Yue Wang, et al
...Results: LIPUS could enhance new periodontal bone formation and bone matrix maturity in FI after GTR treatment. Moreover, clinical assessment and histomorphometric analyses revealed less inflammatory infiltration and superior vascularization within bone grafts in the LIPUS treatment group, indicating the anti-inflammatory and pro-angiogenic effects of LIPUS in FI.
Conclusion: Our investigation on a large animal model demonstrated that LIPUS is a promising adjunctive approach for the regeneration of periodontitis tissue, paving a new avenue for LIPUS application in the field of periodontal regenerative medicine.
https://ieeexplore.ieee.org/document/5118289/
Design and implementation of a Low-power Intensity Pulsed-Ultrasound generator for dental tissue regeneration
Woon Tiong Ang, et al
Abstract: -- This paper presents the design and implementation of a Low-Intensity Pulsed Ultrasound (LIPUS) generator for dental tissue regeneration. It consists of a power supply subsystem, an ultrasonic transducer, an impedance-matching circuit and an integrated circuit consisting of digital controller circuitry and driver circuit. The integrated circuit was designed and fabricated using 0.8µm High-Voltage Technology from Dalsa Semiconductor Inc. The power supply sub-system and impedance matching network are implemented using discrete components. Upon construction, the LIPUS generator was verified to function correctly and is capable of producing LIPUS power upwards of 100mW in the vicinity of the transducer';s resonance frequency. Power efficiency of the circuitry, excluding the power supply sub-system, is estimated at 70%.
https://www.sciencedirect.com/science/article/abs/pii/S0301562916302599
Effect of Low-Intensity Pulsed Ultrasound on a Rat Model of Dentin–Dental Pulp Injury and Repair
Author links open overlay panel
Fei Wang, et al
Abstract -- This study investigated histopathologic changes in dental pulp after treatment with low-intensity pulsed ultrasound (LIPUS). Fifty rats were randomly divided into an experimental group (n = 25) and a blank control group (n = 25). In the experimental group, a cavity was prepared in the bilateral maxillary first molars. The upper right first molars were stimulated with LIPUS (30 mW/cm2, 1.5 MHz) for 20 min/d. The cavities prepared in the left teeth were used as experimental controls (i.e., no LIPUS). Five rats in each group were sacrificed at days 1, 3, 5, 7 and 14. Inflammatory response was visible at different time points after cavity preparation, peaking at day 3, after which it gradually weakened. More reparative dentin was found on the LIPUS treatment side. transforming growth factor-β1 expression increased after treatment, peaking at day 5 and returning to normal at day 14 on both sides, but was stronger with LIPUS treatment. SMAD2 and SMAD3 expressions in the dental pulp gradually increased after cavity preparation, especially in the experimental group. LIPUS promoted the repair of dentin–pulp complex injury, to a certain extent and should be investigated further as a potential therapy.
CN214342694 -- Ultrasonic generator for accelerating movement of orthodontic teeth
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The utility model belongs to the technical field of orthodontic correction, and discloses an ultrasonic generator for accelerating movement of orthodontic teeth, two sides of a shell are respectively provided with a power interface and an ultrasonic transducer, the upper side of the shell is embedded with a display screen and a control switch, and a power adapter, an ultrasonic driving device, a singlechip and a Bluetooth chip are fixed in the shell; the output end of the single-chip microcomputer is electrically connected with the input end of the display screen and the input end of the ultrasonic driving device, the output end of the ultrasonic driving device is electrically connected with the input end of the ultrasonic transducer, the single-chip microcomputer is electrically connected with the Bluetooth chip, and the Bluetooth chip is connected with a mobile phone end APP in a wireless mode. Cells participating in fracture repair are directly influenced by low-intensity pulse ultrasound stimulation, and the low-intensity pulse ultrasound acts on a pressure receptor of a cell membrane, so that some biochemical reactions occur in the cells; the proliferation and calcification of hypertrophy cartilage cells are promoted; the bone reconstruction is influenced, and the bone reconstruction process is promoted.
US8292834 -- ULTRASOUND STIMULATION DEVICES AND TECHNIQUES
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Inventor: EL-BIALY TAREK, et al
Ultrasound stimulation devices and related techniques are disclosed. An ultrasound transducer for generating ultrasound energy is carried by a transducer housing that seals the transducer and may also include a positioning element for positioning the transducer proximate an application area to which generated ultrasound energy is to be applied. The transducer housing may also carry such components as a battery, a wireless receiver, and a controller. The same housing or a separate sensor housing may include an ultrasound sensor that provides feedback to the ultrasound transducer or its controller, illustratively through a wireless transmitter.