rexresearch
Akkermansia muciniphila vs Cancer
https://pmc.ncbi.nlm.nih.gov/articles/PMC12806543/
The role of Akkermansia muciniphila in cancer: Mechanisms, therapeutic potential, and challenges
Xi Chen, et al.
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Abstract -- Akkermansia muciniphila (A. muciniphila), regarded as a promising candidate for next‐generation probiotic applications, predominantly inhabits the intestinal mucus layer, where it plays a crucial role in maintaining gut barrier integrity and modulating immune responses. Recently, the bacterium has been recognized for its ambivalent influence on cancer, impacting both tumor progression and therapeutic interventions. Research indicates that A. muciniphila might possess both tumorigenic and anticancer capabilities, influenced by factors such as the composition of the gut microbiota, dietary modifications, and immune modulation. There is a compelling need for further studies to uncover the precise mechanisms and optimal use of A. muciniphila in oncology and beyond...
https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2023.1303795/full
Akkermansia muciniphila - friend or foe in colorectal cancer?
Ekaterina O. Gubernatorova, et al.
[ PDF ]
https://www.sciencedirect.com/science/article/pii/S0753332224003007
The role of Akkermansia muciniphila in colorectal cancer: A double-edged sword of treatment or disease progression?
Elnaz Faghfuri a, Pourya Gholizadeh
[ PDF ]
Abstract -- Colorectal cancer (CRC) is the second most cancer-related death worldwide. In recent years, probiotics have been used to reduce the potential risks of CRC and tumors with various mechanisms. Different bacteria have been suggested to play different roles in the progression, prevention, or treatment of CRC. Akkermansia muciniphila is considered a next-generation probiotic for preventing and treating some diseases. Therefore, in this review article, we aimed to describe and discuss different mechanisms of A. muciniphila as an intestinal microbiota or probiotic in CRC. Some studies suggested that the abundance of A. muciniphila was higher or increased in CRC patients compared to healthy individuals. However, the decreased abundance of A. muciniphila was associated with severe symptoms of CRC, indicating that A. muciniphila did not play a role in the development of CRC. In addition, A. muciniphila administration elevates gene expression of proliferation-associated molecules such as S100A9, Dbf4, and Snrpd1, or markers for cell proliferation. Some other studies suggested that inflammation and tumorigenesis in the intestine might promoted by A. muciniphila. Overall, the role of A. muciniphila in CRC development or inhibition is still unclear and controversial. Various methods of bacterial supplementation, such as viability, bacterial number, and abundance, could all influence the colonization effect of A. muciniphila administration and CRC progression. Overall, A. mucinipila has been revealed to modulate the therapeutic potential of immune checkpoint inhibitors. Preliminary human data propose that oral consumption of A. muciniphila is safe, but its efficacy needs to be confirmed in more human clinical studies.
PATENTS
CN122629153 [ Transl. ] - Starch-based feed for improving abundance of Akkermansia muciniphila
The invention discloses a starch-based feed for improving the abundance of Akkermansia muciniphila bacteria, and belongs to the technical field of functional foods. Experimental results show that the starch-based feed can significantly reverse the flora disorder of mice: PCoA analysis shows that the starch-based feed can restore the whole structure of the disordered flora to a healthy state; according to the present invention, the regulation effect is specifically represented by improving the disordered ratio of phylum firmicalis/phylum bacteroides, promoting the proliferation of the beneficial bacterium Akkermansia muciniphila, and inhibiting the growth of conditioned pathogens, and the regulation effect is dose-dependent. Therefore, the modified starch-based feed is a functional food raw material with potential for regulating intestinal flora.
US20250179423 - POTENT HIGH YIELD FERMENTATION AND MANUFACTURING OF AKKERMANSIA MUCINIPHILA USING PLANT-BASED MUCIN AND ENCAPSULATION FOR STABILITY
Provided herein are systems and methods for Potent high yield fermentation and Manufacturing of Akkermansia muciniphila using plant-based Mucin and encapsulation for better stability.
KR20240173904 - Culture media for producing high yield of Akkermansia sp. culturing method and freeze drying method using it
The present invention relates to a medium composition for culturing Akkermansia sp. microorganisms comprising N-acetylhexosamine, L-threonine, and a yeast extract, wherein the medium composition comprises disaccharides as a single carbon source and an animal protein hydrolysate as a nitrogen source, and a method for culturing Akkermansia sp. microorganisms using the same. The medium composition can culture Akkermansia sp. microorganisms such as Akkermansia muciniphila, which is a fastidious anaerobic microorganism, at a high yield, and can manufacture freeze-dried cell powder with improved survival rate and stability for the cultured Akkermansia sp. microorganisms.
JP2024038952 - AKKERMANSIA BACTERIA GROWTH PROMOTER
To provide an Akkermansia bacteria growth promoter that can promote the growth of Akkermansia bacteria, and an intestinal environment improving composition comprising the same.SOLUTION: An Akkermansia bacteria growth promoter comprises at least one selected from the group consisting of Rosmarinus officinalis extract, chitinoligosaccharide, propolis extract, Cyclolepis genistoides D. Don extract, Cinnamomum cassia Blume extract and rice oil extract. Preferably, Akkermansia bacteria is Akkermansia muciniphila. Preferably, Akkermansia bacteria growth promoter is applied for anti-obesity purposes. There is also provided an intestinal environment improving composition comprising the Akkermansia bacteria growth promoter.
EP4814105 - CULTURE MEDIUM AND METHOD FOR CULTURING AKKERMANSIA MUCINIPHILA
Disclosed are a method for optimizing a bacterial culture medium based on a metabolic network model, a culture medium for Akkermansia muciniphila obtained thereby, and related applications thereof. The medium optimized by the method reduces redundant components present in the prior art media, has a simple composition and low cost, and can more directly and effectively promote high-density culture of bacteria. The resulting culture medium for Akkermansia muciniphila can increase the viable cell count to above 10<sup>10</sup> cfu/mL, enabling industrial production of Akkermansia muciniphila.
JP2023098030 [ Transl. ] - COMPOSITION FOR PROMOTING PROLIFERATION OF AKKERMANSIA MUCINIPHILA COMPRISING DIETARY FIBER
To provide means for efficiently promoting the proliferation of Akkermansia muciniphila, known as slimming bacteria, in human enteric bacteria.SOLUTION: This invention has been completed with the findings that dietary fibers generally promote the proliferation of Akkermansia muciniphila
JP2023096870 [ Transl. ] - AKKERMANSIA BACTERIA GROWTH PROMOTER
To provide a novel Akkermansia muciniphila growth promoter, and to provide a novel agent for improving or alleviating diseases or conditions that can be improved or alleviated by ingesting or administering Akkermansia muciniphila.SOLUTION: According to the present invention, provided is an Akkermansia muciniphila growth promoter containing cacao polyphenol as an active ingredient. According to the present invention, also provided is an agent for improving or al9leviating diseases or conditions that can be improved or alleviated by ingesting or administering Akkermansia muciniphila, containing cacao polyphenols as an active ingredient. The cocoa polyphenol preferably contains 25 mass% or more of mono- to tetra-meric polyphenols.
US20250057893 - PHARMACEUTICAL COMPOSITION FOR PREVENTING OR TREATING CANCER OR INFLAMMATORY DISEASE
The present invention relates to a medium composition for culturing microorganisms of the genus Akkermansia, which includes N-Acetylhexosamine, L-Threonine, and a yeast extract, and to a medium composition for culturing microorganisms of the genus Akkermansia, which includes disaccharides as a sole carbon source and animal protein hydrolyzate as a nitrogen source and a method for culturing microorganisms of the genus Akkermansia using the same. The present invention enables the culturing of microorganisms of the genus Akkermansia such as Akkermansia muciniphila, an unculturable anaerobic microorganism, with high yield and enables the production of freeze-dried bacterial powder with improved survival rate and stability for the cultured microorganisms of the genus Akkermansia.
CN115851529 [ Transl. ] - Akkermansia muciniphila improved culture medium
The invention belongs to the technical field of bacterial culture media, and particularly relates to an improved culture medium of Akkermansiamuciniphila. The invention discloses an Akkermansiamuciniphila improved culture medium. The Akkermansiamuciniphila improved culture medium is prepared from a yeast extract, EGCG (epigallocatechin gallate), monopotassium phosphate, disodium hydrogen phosphate, sodium chloride, glucose, N-acetyl-glucosamine, N-acetyl-D-galactosamine, urea, threonine, proline, L-cysteine hydrochloride, sodium pyruvate, ferric pyrophosphate and vitamin K3 (N-acetyl-D-galactosamine and urea can not be added). The improved culture medium is composed of plant source components, does not contain animal source components, is high in safety, not only can maintain excellent in-vitro growth of A.muciniphila, but also does not change the normal form of thalli, and is suitable for in-vitro large-volume culture of A.muciniphila.
CN115141778 [ Transl. ] - Method for specifically screening Ackermania muciniphila by using composite regulating agent
Akkermansia muciniphila (Akk bacteria for short) is a class of potential probiotics which are recognized in the world, but the requirements on nutrition are very strict, and the isolated culture is extremely difficult, so that the progress of isolated culture, research and development of the bacteria is slow. The invention provides a method for specifically screening Akk bacteria by using a composite regulating agent, and belongs to the technical field of microorganisms. The composite regulating agent comprises a regulating agent A, a regulating agent B and a regulating agent C. An adopted culture medium is a synthetic culture medium which is improved on the basis of a brain heart infusion broth culture medium (Brain Heart Infusion, abbreviated as BHI) or can be used for culturing Akk bacteria. The composite regulating agent culture medium can simultaneously inhibit gram-positive bacteria and gram-negative bacteria in an excrement sample, basically has no inhibiting effect on Akk bacteria, and also has a certain growth promoting effect, so that the Akk bacteria can well grow on the surface of a flat plate, the screening difficulty of the Akk bacteria in a complex mixed sample is reduced, and the screening efficiency is improved. And the method has important significance on screening and culturing of the Akk bacteria.
CN120683114 [ Transl. ] - Promoter P1 for ackermania mucophila exogenous gene expression, recombinant vector, recombinant bacteria and application thereof
The invention discloses a promoter P1 for ackermania mucophila exogenous gene expression, a recombinant vector, a recombinant bacterium and application of the promoter P1 and the recombinant bacterium. Belongs to the technical field of gene engineering. According to the application, the strong promoter capable of being used for the expression of the ackermania mucophila exogenous gene is screened for the first time, and the strong promoter has super-strong expression quantity. According to the invention, a recombinant plasmid capable of inserting a Lux CDABE report system into Ackerman muciniphila is constructed at the same time, a method for recombining the Lux CDABE report system into an Ackerman muciniphila genome by using a double exchange mode is established on the basis, and a luminous Akkermansia muciniphila SDLYAKK8-Lux strain is successfully constructed, so that the luminous Akkermansia muciniphila SDLYAKK8-Lux strain can be used for preparing the recombinant plasmid for the Ackerman muciniphila, and the luminous Akkermansia muciniphila SDLYAKK8-Lux strain can be used for preparing the Akkerman muciniphila SDLYAKK8-Lux strain. Research finds that the luminous intensity of the strain is strongly related to the number of the strains, and the blank that no living body fluorescence imaging construction method for the AKK bacteria exists is filled up.
US20250195563 - METHOD FOR GROWTH OF AKKERMANSIA MUCINIPHILA COMPRISING ADMINISTERING PLATINUM NANOPARTICLES
This invention is to provide the novel composition that grows Akkermansia muciniphila in the intestinal microflora. The composition for the growth of Akkermansia muciniphila containing platinum nanoparticles as an active ingredient.
CN113322202 [ Transl. ] - Akkermansia muciniphila as well as culture method and application thereof
The invention discloses akkermansia muciniphila. The akkermansia muciniphila is classified and named as Akkermansia muciniphila JWA48, and is preserved in the China Center for Type Culture Collection (CCTCC) on April 16, 2021, and the preservation number is CCTCC M 2021388. According to the invention, the akkermansia muciniphila is obtained through strain screening, and has the advantages of fast growth, high yield and relatively low requirements on culture conditions. Compared with commercially available akkermansia muciniphila, the maximum biomass of the akkermansia muciniphila is increased by about 50%, and the akkermansia muciniphila can tolerate O2 concentration of 0.5% or below.
TW202116341 - Composition for promoting growth of Akkermansia muciniphila and uses thereof
The present disclosure provides a composition for promoting growth of Akkermansia muciniphila. Wherein the composition comprises Pachyrhizus erosus ferment. The composition further promotes the growth of Parabacteroides goldsteinii and Bifidobacterium in the user, and can modulate the user's weight, body fat, waist circumference, and skin condition.
CN112342151 [ Transl. ] - Anaerobic culture method of akkermansia muciniphila
The invention belongs to the technical field of microbial culture, and particularly discloses an anaerobic culture method of akkermansia muciniphila. Mixed gas adopted by anaerobic culture comprises 85-94% of N2, 5% of H2 and 1-10% of CO2, an adopted culture medium is an improved BHI culture medium, and Mucin and Yeast Excact are added into the improved BHI culture medium. The culture medium and culture conditions are matched, so that growth and propagation of the Akk bacteria are not limited by the height of the liquid level, the Akk bacteria can well grow in a shake flask and can also well grow on the surface of a flat plate, the culture difficulty of the Akk bacteria is reduced, the growth speed of the Akk bacteria is reduced to 24-36 hours from 3-4 days in the prior art, the culture time is greatly shortened, the activity of the Akk bacteria is improved, the growth of the Akk bacteria is promoted, and the method has important significance in expanded culture and deep research of the Akk bacteria.
CN110551658 [ Transl. ] - Low-oxygen culture method for akkermansia muciniphila
The invention discloses a low-oxygen culture method for akkermansia muciniphila. In the low-oxygen culture method, a low-oxygen gas environment is mainly created; a volume ratio of various componentsof mixed gas is as follows: 70-94 percent of N2, 1-5 percent of O2 and 5-25 percent of CO2; and the culture time is 24-36h. The invention provides a brand-new low-oxygen mixed gas atmosphere which isrelatively high in oxygen concentration and more suitable for growth of Akk bacteria. Compared with a traditional absolute oxygen-free environment and an existing micro-oxygen environment, by adoptingthe method disclosed by the invention, the time of Akk bacterium culture, entering a stabilization period, is greatly shortened, and the low-oxygen environment can also show a similar promotion effect for different culture media.
CN110079474 - Method for high-density culture of Akkermansia muciniphila
The invention belongs to the field of microbial fermentation, relates to a method for high-density culture of Akkermansia muciniphila and specifically relates to the method for high-density culture of Akkermansia muciniphila by utilizing a pure element culture medium by replacing natural composition nitrogen sources (such as peptone and yeast extract) in the culture medium with a specific amino acid combination. According to the method provided by the invention, a large amount of Akk.Muciniphila is acquired at low cost. The culture medium is a synthetic medium, the nitrogen source compositionis allergen-free and contains no unknown component, and all the raw materials meet Food Additives Use Regulations of Food Safety National Standard (GB2760-2017), so that the method is suitable for industrial production of food and drug-grade Akk.Muciniphila.
CN109810931 [ Transl. ] - Culture medium for culturing akkermansia muciniphila and use method thereof
The invention provides a culture medium for culturing akkermansia muciniphila and a use method thereof, and belongs to the technical field of microorganism culture mediums. Components of the culture medium include soy peptone, threonine, glucose, N-acetylglucosamine, sodium chloride and disodium hydrogen phosphate. By adopting the culture medium, the akkermansia muciniphila quantity required by functional studying of the akkermansia muciniphila can be reached through culture, the components of the culture medium have safe sources, and potential biological hazards do not exist.
WO2019092896 - AKKERMANSIA MUCINIPHILA PROLIFERATION MATERIAL
The present invention addresses the problem of providing a novel proliferation material which enables the proliferation of Akkermansia muciniphila. An Akkermansia muciniphila proliferation material containing at least one polyphenol component selected from the group consisting of quercetin glycoside, isorhamnetin, cyanidin glucoside, hesperetin, hesperidin, apigenin, chrysin, tangeretin, nobiletin and tannic acid.
US12077761 -- SYSTEMS AND METHODS FOR GENETIC MANIPULATION OF AKKERMANSIA SPECIES
The present disclosure provides methods and systems for genetically altering and screening Akkermansia bacteria, including Akkermansia muciniphila. The methods also provide genetically altered bacteria, libraries of genetically altered bacteria and use of such bacteria for treatment of diseases.
CN111304133 [ Transl. ] - Culture medium for culturing Akkermansia muciniphila, and application method and applications of culture medium
The invention provides a culture medium for culturing Akkermansia muciniphila, and an application method and applications of the culture medium. The culture medium includes brain heart infusion, porcine gastric mucin, L-cysteine hydrochloride, threonine, N-acetyl-D-glucosamine and vitamins. Through the adding of the L-cysteine hydrochloride, the threonine, the N-acetyl-D-glucosamine and the vitamins in the culture medium, the Akkermansia muciniphila can still obtain enough nutrients in the culture medium with low concentrations of the brain heart infusion and the porcine gastric mucin, so thatthe normal growing and reproduction of strains can be guaranteed. After the Akkermansia muciniphila is cultured by the provided culture medium, the OD value of the obtained bacterial solution is 0.8211-0.8741.
JP2019043866 [ Transl. ] - COMPOSITION FOR PROMOTING INCREASE OF AKKERMANSIA MUCINIPHILA
To provide an unconventionally new composition for promoting increase of Akkermansia muciniphila, and a food, pharmaceutical, and feed containing the composition.SOLUTION: The present invention provides a composition for promoting increase of Akkermansia muciniphila, containing κ-casein glycomacropeptide (GMP) as an active ingredient. There are also provided a food and drink, pharmaceutical, and feed for promoting increase of Akkermansia muciniphila, containing GMP as an active ingredient.
CN108403930 [ Transl. ] - Compound preparation for increasing ratio of AKK (akkermansia muciniphila) bacteria and pathogenic bacteria in intestines as well as preparation method and application thereof
The invention provides a compound preparation for increasing ratio of AKK (akkermansia muciniphila) bacteria and pathogenic bacteria in intestines as well as a preparation method and application thereof, and relates to the field of oral preparations. The compound preparation for increasing the ratio of AKK bacteria to pathogenic bacteria in the intestines is prepared from the following raw materials in percentage by mass: 21% to 64% of oligosaccharide, 21% to 44% of plant extract, 9% to 31% of fungus extract, 0.5% to 5% of sodium taurate, and 0.5% to 5% of elaeagnus angustifolia extract. The compound preparation has the advantages that the safety is good, and the intestinal flora can be effectively regulated. The preparation method of the compound preparation comprises the following stepsof mixing the oligosaccharide, the plant extract, the fungus extract, sodium taurate and the elaeagnus angustifolia extract, and grinding, so as to obtain the compound preparation. The preparation method has the advantages that the technology is simple, and the industrialized production is easy. The application of the compound preparation for increasing the ratio of AKK bacteria to pathogenic bacteria in the intestines is characterized in that the compound preparation is used for preparing the oral preparation for increasing the abundance of the AKK bacteria in the intestines, and reducing theabundance of the pathogenic bacteria.
US10988509 - Method of culturing Akkermansia
A method for cost-effectively and efficiently culturing Akkermansia muciniphilais is disclosed. High biomass yields can be obtained on chemically defined media. This allows for large scale production of A. muciniphila suitable for use in humans, such as for pharmaceutical or food applications. The A. muciniphila can be produced free of animal-derived products, thereby allowing a broad-range of applications.
CN111763653 [ Transl. ] - Culture method of Akkermansia muciniphila based on novel electron acceptor
The invention discloses a culture method of Akkermansia muciniphila based on a novel electron acceptor. The culture method comprises the following steps: adding nitrate or a TMAO solution into a BHI culture medium, inoculating Akkermansia muciniphila (Akk bacteria) into the culture medium, and performing culturing to obtain the Akkermansia muciniphila under the culture conditions that the ratio ofthe mixed gas is 80-95% of N2, 5-20% of H2 and 0-3% of O2. The nitrate or TMAO solution is adopted to replace traditional gaseous CO2 to serve as an electron acceptor of the Akk bacteria, and the influence of CO2 concentration fluctuation on the growth of the Akk bacteria is avoided; meanwhile, the nitrate or TMAO solution can also enhance the tolerance of the Akk bacteria to oxygen, the inhibition effect of oxygen on the Akk bacteria is avoided, and the growth of the Akk bacteria can be promoted within a certain oxygen concentration range, so that the Akk bacterium culture method which is lower in cost, higher in controllability, more loose in culture condition and higher in yield is obtained, and a new idea is provided for culture of Akk bacteria.
CN111938158 [ Transl. ] - Composition for preventing reduction of abundance of Akkermansia muciniphila in intestinal tract
The invention relates to a composition for regulating the abundance of Akkermansia muciniphila in the intestinal tract. The composition is composed of L-valine and preparation auxiliary materials. Theeffect of preventing the reduction of the abundance of the Akkermansia muciniphila in the intestinal tract is achieved by supplementing a certain amount of L-valine to the intestinal tract. Generally, the content of the Akkermansia muciniphila in the body of a patient suffering from inflammatory bowel disease and obesity can be reduced, and the composition can restore the abundance of the Akkermansia muciniphila in the mode of supplementing the L-valine, so that the metabolic state is improved. The composition can serve as food or a health care product or a medicine for restoring the abundance of the Akkermansia muciniphila.
US2021023159 - Method for promoting growth of Akkermansia muciniphila using musa ferments
Provided is a method of promoting the growth of Akkermansia muciniphila, including contacting A. muciniphila with an effective amount of a prebiotic composition including a Musa ferment. The prebiotic composition promotes the growth of A. muciniphila in the intestine, and reduces the body weight, fat percentage, waist circumference, and hip circumference of obese individuals.