rexresearch
Dandelion vs Cancer
Related : Sirayam PANDEY, et al. : Dandelion & Long Pepper Fruit vs Cancer
https://www.bitchute.com/video/qgvYCZNVqvYs
Dandelion root eliminates 95% of cancer cells in the lab
https://www.oncotarget.com/article/11485/text/
Oncotarget. 2016; :73080-73100.
https://doi.org/10.18632/oncotarget.11485
Dandelion root extract affects colorectal cancer proliferation and survival through the activation of multiple death signalling pathways
P. Ovadje, et al
[ PDF ]
ABSTRACT -- Dandelion extracts have been studied extensively in recent years for its anti-depressant and anti-inflammatory activity. Recent work from our lab, with in-vitro systems, shows the anti-cancer potential of an aqueous dandelion root extract (DRE) in several cancer cell models, with no toxicity to non-cancer cells. In this study, we examined the cancer cell-killing effectiveness of an aqueous DRE in colon cancer cell models. Aqueous DRE induced programmed cell death (PCD) selectively in > 95% of colon cancer cells, irrespective of their p53 status, by 48 hours of treatment.
The anti-cancer efficacy of this extract was confirmed in in-vivo studies, as the oral administration of DRE retarded the growth of human colon xenograft models by more than 90%. We found the activation of multiple death pathways in cancer cells by DRE treatment, as revealed by gene expression analyses showing the expression of genes implicated in programmed cell death. Phytochemical analyses of the extract showed complex multi-component composition of the DRE, including some known bioactive phytochemicals such as α-amyrin, β-amyrin, lupeol and taraxasterol. This suggested that this natural extract could engage and effectively target multiple vulnerabilities of cancer cells. Therefore, DRE could be a non-toxic and effective anti-cancer alternative, instrumental for reducing the occurrence of cancer cells drug-resistance.
https://link.springer.com/article/10.1007/s42452-024-06419-7
Antioxidant and antimicrobial activities of Dandelion root extract (Taraxacum officinale) and its cytotoxic effect on MDA-MB-231
Ridwan Olanrewaju Shittu, Ismaila Ceesay & Pwadubashiyi Coston Pwavodi
[ PDF ]
Abstract -- Breast cancer (BC) is one of the leading causes of death globally, resulting in 2.26 million cases worldwide. Treatments to downregulate these tumor cells include surgery, immunotherapy, and chemotherapy. However, habituation is expressed by the tumor cells after using the latter. Different plants have been used to treat diseases ranging from bacterial infection to cancer. Natural health products confirmed to be potent and still undergoing clinical trials include the dandelion plant, also known as Taraxacum officinale. This study evaluated Dandelion root extract's (DRE) antioxidant, antimicrobial, and cytotoxic effects. The Gas Chromatography-Mass Spectrometry (GC–MS) result revealed the presence of bioactive compounds such as P-hydroxybenzoic, chlorogenic acid, vanillic acid, caffeic acid, lupeol, leutolin, apigenin, coumestrol and much more that are present in the DRE extract. Antioxidant activity using a diphenylpicryl hydrazine (DPPH) scavenging assay was employed, and the result suggested a stable radical IC50 value of 2.860 ± 0.001 mg/mL. Trypan blue exclusion assay was used to determine the cytotoxic effect on MDA-MB-231 cells. Antimicrobial activity was assessed by disc diffusion assay, and the average diameter values obtained were 25 mm for gentamicin. No antimicrobial activity was observed for the extracts at different concentrations. DRE showed no inhibitory effect against two gram-negative and gram-positive bacteria pathogens. Our study indicates that DRE has proven novel in killing breast cancer cells, has a mildly vigorous antioxidant activity to scavenge free radicals, and has no inhibitory effect against some bacteria.
https://www.sciencedirect.com/science/article/pii/S075333221731987X
Dandelion root extract suppressed gastric cancer cells proliferation and migration through targeting lncRNA-CCAT1
Huanhuan Zhu , et al.
Abstract -- Gastric cancer (GC) is one of the most common tumors worldwide. Standard treatment after early detection involves surgical excision (recurrence is possible), and metastatic gastric cancer is refractory to immuno-, radio-, and most harmful chemotherapies. Various natural compounds have shown efficacy in killing different cancers, albeit not always specifically. In this study, we show that dandelion root extract (DRE) specifically and effectively suppresses proliferation and migration in human gastric cells without inducing toxicity in noncancerous cells. Long noncoding RNAs (lncRNAs) are known to promote tumorigenesis in many cancer types. Here, we showed that the lncRNA colon cancer-associated transcript-1 (CCAT1) was down-regulated in dandelion-treated GC cells. Furthermore, downregulation of CCAT1 inhibited proliferation and migration of gastric cells. We also found that DRE exerted its function in GC cells partially through targeting CCAT1. This data will provide a basis on which further research in cancer treatment through DRE can be executed.
https://pubmed.ncbi.nlm.nih.gov/37700002/
https://www.nature.com/articles/s41598-023-42177-z
Combined dandelion extract and all-trans retinoic acid induces cytotoxicity in human breast cancer cells
Hamed Rezaie, et al.
[ PDF ]
Abstract -- Breast cancer is one of the most prevalent and deadly cancers among women worldwide. Recently, natural compounds have been widely used for the treatment of breast cancer. Present study evaluated antiproliferative and anti-metastasis activities of two natural compounds of dandelion and all-trans-retinoic acid (ATRA) in human MCF-7 and MDA-MB231 breast cancer cells. We also evaluated the expression of MMP-2, MMP-9, IL-1β, p53, NM23 and KAI1 genes. Data showed a clear additive cytotoxic effect in concentrations of 40 μM ATRA with 1.5 and 4 mg/ml of dandelion extract in MCF-7 and MDA-MB231 cells, respectively. In both cell lines, compared with the untreated cells, the expression levels of MMP-9 and IL-1β were significantly decreased while p53 and KAI1 expression levels were increased. Besides, MMP-2 and NM23 had different expressions in the two studied cell lines. In conclusion, dandelion/ATRA co-treatment, in addition to having strong cytotoxic effects, has putative effects on the expression of anti-metastatic genes in both breast cancer cells.
https://onlinelibrary.wiley.com/doi/10.1155/2019/2951428
Dandelion Root and Lemongrass Extracts Induce Apoptosis, Enhance Chemotherapeutic Efficacy, and Reduce Tumour Xenograft Growth In Vivo in Prostate Cancer
Christopher Nguyen, et al.
[ PDF ]
Abstract -- Many conventional chemotherapies have indicated side effects due to a lack of treatment specificity and are thus not suitable for long-term usage. Natural health products are well-tolerated and safe for consumption, and some have pharmaceutical uses particularly for their anticancer effects. We have previously reported the anticancer efficacy of dandelion (Taraxacum officinale) root and lemongrass (Cymbopogon citratus) extracts. However, their efficacy on prostate cancer and their interactions with standard chemotherapeutics have not been studied to determine if they will be suitable for adjuvant therapies. If successful, these extracts could potentially be used in conjunction with chemotherapeutics to minimize the risk of drug-related toxicity and enhance the efficacy of the treatment. We have demonstrated that both dandelion root extract (DRE) and lemongrass extract (LGE) exhibit selective anticancer activity. Importantly, DRE and LGE addition to the chemotherapeutics taxol and mitoxantrone was determined to enhance the induction of apoptosis when compared to individual chemotherapy treatment alone. Further, DRE and LGE were able to significantly reduce the tumour burden in prostate cancer xenograft models when administered orally, while also being well-tolerated. Thus, the implementation of these well-tolerated extracts in adjuvant therapies could be a selective and efficacious approach to prostate cancer treatment.
https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2022.897465/full
Front. Pharmacol., 19 May 2022
Sec. Pharmacology of Anti-Cancer Drugs Volume 13 - 2022 | https://doi.org/10.3389/fphar.2022.897465
Dandelion Seed Extract Affects Tumor Progression and Enhances the Sensitivity of Cisplatin in Esophageal Squamous Cell Carcinoma
Wenqing Guo , et al.
[ PDF ]
Abstract -- Like dandelion, dandelion seed also have anti-inflammatory activity. Therefore, in this article, we intend to explore the anti-cancer availability of aqueous dandelion seed extract (DSE) in esophageal squamous cell carcinoma (ESCC). Firstly, the effects of DSE on cell proliferation, apoptosis, migration, invasion and angiogenesis were investigated. Then to explore the mechanism of DSE against ESCC, the levels of proliferation-associated proteins (PI3K, Akt and pAkt), apoptosis-associated proteins (survivin, Bcl-2, Bax, caspase3 and caspase9), metastasis-associated proteins (MMP2, MMP9, VEGF) and EMT progression-associated proteins (Snail, E-cadherin and Vimentin) were analyzed. Next, we further explored the effect of DSE on the sensitivity of cisplatin (DDP) in ESCC cells and investigated the effect of DSE combined with DDP on DNA damage repair-associated proteins (MSH2, MLH1 and ERCC1) and drug resistant target protein STAT3. The results indicated that DSE selectively inhibited cell growth, proliferation, migration, invasion, angiogenesis and induced cell apoptosis in ESCC cells. It was observed the decreased PI3K, Akt and pAkt proteins levels in KYSE450 and Eca109 cells administrated with DSE. The data also showed that the application of DSE decreased the level of survivin and the ratio of Bcl-2/Bax, while increased the levels of caspase3 and caspase9. We also observed that DSE significantly decreased the levels of MMP2, MMP9 and VEGF proteins and inhibited the EMT progression in KYSE450 and Eca109 cells. In addition, survivin plays a critical role in DSE against ESCC followed with the application of survivin inhibitor YM155 impairing the inhibitory abilities of DSE in ESCC cells. Meanwhile, it was observed that DSE enhances the sensitivity of DDP to human ESCC cells via promoting DNA damage and inhibiting phosphorylation of STAT3. Therefore, DSE may affect ESCC progression and enhance the sensitivity of cisplatin, and consequently become an effective anti-cancer option for human ESCC treatment.
http://espacenet.com/
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